pkcαcat vector Search Results


pkcαcat vector  (Addgene inc)
90
Addgene inc pkcαcat vector
(A) Representative immunoblot of OVCAR8 RFP and OVCAR8 RFP PAX8 −/− clone 1 and clone 2 demonstrates PAX8 deletion reduces PKCα levels. (B) Wound closure assay performed with OVCAR8 RFP and OVCAR8 RFP PAX8 −/− clone 1 and clone 2 exposed to DMSO (control) or 5nM Go6976 (experimental) inhibitor against PKCα. (C) Wound closure assay performed over 24 hours with OVCAR8 RFP and OVCAR8 RFP PAX8 −/− clone 1 and clone 2 transfected with neomycin vector (control) or <t>PKCαCAT</t> vector (experimental) containing the PKCα catalytic site. Error bars for all assays represent standard errors for three replicates (n=3, error bars = SEM).
Pkcαcat Vector, supplied by Addgene inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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pkcαcat vector - by Bioz Stars, 2026-03
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(A) Representative immunoblot of OVCAR8 RFP and OVCAR8 RFP PAX8 −/− clone 1 and clone 2 demonstrates PAX8 deletion reduces PKCα levels. (B) Wound closure assay performed with OVCAR8 RFP and OVCAR8 RFP PAX8 −/− clone 1 and clone 2 exposed to DMSO (control) or 5nM Go6976 (experimental) inhibitor against PKCα. (C) Wound closure assay performed over 24 hours with OVCAR8 RFP and OVCAR8 RFP PAX8 −/− clone 1 and clone 2 transfected with neomycin vector (control) or PKCαCAT vector (experimental) containing the PKCα catalytic site. Error bars for all assays represent standard errors for three replicates (n=3, error bars = SEM).

Journal: Oncogene

Article Title: Proteomic analysis reveals a role for PAX8 in peritoneal colonization of high grade serous ovarian cancer that can be targeted with micelle encapsulated thiostrepton

doi: 10.1038/s41388-019-0842-2

Figure Lengend Snippet: (A) Representative immunoblot of OVCAR8 RFP and OVCAR8 RFP PAX8 −/− clone 1 and clone 2 demonstrates PAX8 deletion reduces PKCα levels. (B) Wound closure assay performed with OVCAR8 RFP and OVCAR8 RFP PAX8 −/− clone 1 and clone 2 exposed to DMSO (control) or 5nM Go6976 (experimental) inhibitor against PKCα. (C) Wound closure assay performed over 24 hours with OVCAR8 RFP and OVCAR8 RFP PAX8 −/− clone 1 and clone 2 transfected with neomycin vector (control) or PKCαCAT vector (experimental) containing the PKCα catalytic site. Error bars for all assays represent standard errors for three replicates (n=3, error bars = SEM).

Article Snippet: Transient transfection of OVCAR8 RFP cell lines was performed with either 2.5μg of CMV-PAX8 vector (Transomic, Huntsville, AL, Catalog No. TCM1204) or 2.5μg of PKCαCAT vector (generated by Dr. Bernard Weinstein, Addgene plasmid #21234).

Techniques: Western Blot, Wound Closure Assay, Control, Transfection, Plasmid Preparation